According to the RSPCA, Gloucestershire has been rumoured to be pulling from the cull and has called on Westminster to hear the clear message and halt the cull.
The cull is due to start on 1 June in both Gloucestershire and Somerset. RSPCA chief executive Gavin Grant said: "We are very intrigued to hear rumours that Gloucestershire has dropped out of this senseless slaughter. If true, the message could not be more loud or clear.
"Local people do not want this cull. Defra ministers must not ignore scientific facts and local and parliamentary opinion any longer. They must drop flawed plans to kill large numbers of badgers.
"Culling is not the answer to the problems of Bovine TB in cattle, vaccination of wildlife and better biosecurity on farms is the way forward. The cows, as well as the badgers, need our help."